Base barrier cells provide compartmentalization of choroid plexus, brain and CSF

Additional supplemental information paper: 1. RNAmarkers numbered clusters CNS merged dataset (Extended Data Figure 2a)RNA markers for the numbered clusters of the CNS vascular-associated and glial cell merged scRNA-Seq dataset. Marker genes were determined by differential expression analysis for each cluster versus all other clusters. This was performed by running the Seurat function FindAllMarkers. See extended materials and methods for details. 2. Automatic annotation ScType custom markerfile (Extended Data Figure 2b)Custom marker file, created by exploring various brain single cell atlases, which was used to automatically annotate the cell populations in the CNS vascular-associated and glial cell merged scRNA-Seq dataset. 3. RNAmarkers numbered clusters FB subset of CNS merged dataset (Extended Data Figure 3a)RNA markers for the numbered clusters of the CNS fibroblast subset dataset. Marker genes were determined by differential expression analysis for each cluster versus all other clusters. This was performed by running the Seurat function FindAllMarkers. See extended materials and methods for details. 4. RNAmarkers annotated clusters ChP FB age dataset (Extended Data Figure 4c)RNA markers for the manually annotated clusters of the dataset consisting of ChP fibroblasts of E16, 7-, 22- and 82-week-old mice. Marker genes were determined by differential expression analysis for each cluster versus all other clusters. This was performed by running the Seurat function FindAllMarkers. See extended materials and methods for details.