DETERMINATION OF PEROXIDASE ACTIVITY USING STOP REAGENT

A number of substances inhibiting the activity of horseradish peroxidase have been studied. The most effective "stop reagents" are hydrochloric acid and concentrated hydrogen peroxide solution. A method for determining the activity of plant peroxidases by sampling using ortho-phenylenediamine as a reducing substrate and concentrated hydrogen peroxide solution as a stop reagent has been optimized. Keywords: peroxidase, ABTS, spectrophotometry, photocolorimetry, ortho-phenylenediamine.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2024-09-02
DOI
https://doi.org/10.5281/zenodo.13758229
Primary Topic
Enzyme-mediated dye degradation
Type
article
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article

DETERMINATION OF PEROXIDASE ACTIVITY USING STOP REAGENT

Zenodo (CERN European Organization for Nuclear Research)
Enzyme-mediated dye degradation
article

DETERMINATION OF PEROXIDASE ACTIVITY USING STOP REAGENT

article en

Abstract

A number of substances inhibiting the activity of horseradish peroxidase have been studied. The most effective "stop reagents" are hydrochloric acid and concentrated hydrogen peroxide solution. A method for determining the activity of plant peroxidases by sampling using ortho-phenylenediamine as a reducing substrate and concentrated hydrogen peroxide solution as a stop reagent has been optimized. Keywords: peroxidase, ABTS, spectrophotometry, photocolorimetry, ortho-phenylenediamine.

Zenodo (CERN European Organization for Nuclear Research)
Donetsk State University of Management (UA)
Openalex Percentile: Top 31%
Enzyme-mediated dye degradation
DOIOpen Access PDFOpenAlex
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DETERMINATION OF PEROXIDASE ACTIVITY USING STOP REAGENT · Zenodo (CERN European Organization for Nuclear Research) (2024) | TGRS Research Map | TGRS