Multires-ML-Microscopy: An Open-Source Pipeline for Automated Cell Segmentation and Analysis

Multires-ML-Microscopy is an open-source MATLAB library offering an end-to-end image-processing pipeline for biological cell images. It integrates multilevel wavelet denoising, deep-learning based segmentation with a Mask R-CNN backbone, and multi-object tracking to deliver robust and automated cell detection. A graphical user interface streamlines operation—from data import through parameter tuning to quantitative metric export—making advanced microscopy analysis accessible without requiring expert coding knowledge.