Studies on the Evaluation of Flavonoids (Morin, Naringin, Quercetin & Rutin) in Pongamia Pinnata (L.) Pierre Leaves By HPLC

Pongamia pinnata (L.) Pierre is recognized for its bioactive potential due to the presence of polyphenolic compounds, notably flavonoids, known for their antioxidant properties and associated health benefits. This study focused on the evaluation and quantification of specific flavonoids—Morin, Naringin, Quercetin, and Rutin—in the leaves of P. pinnata. High-Performance Liquid Chromatography (HPLC) was employed for qualitative analysis, utilizing a reverse-phase column with 0.1% phosphoric acid and acetonitrile in a 25:75 ratio. The standard retention times for Quercetin, Morin, Naringin, and Rutin were recorded as 2.513 min, 3.887 min, 7.130 min, and 4.780 min, respectively, with specific findings for Pongamia pinnata leaves indicating Morin at 3.547 min, Rutin at 4.853 min, Naringin at 6.507min, and Quercetin at 2.527 min. The peak areas for these compounds reflected their relative concentrations, underscoring the medicinal value of P. pinnata as a significant source of bioactive flavonoids for pharmacological research