REMEDi4ALL - Standard Operating Procedure - A high-content screening assay to evaluate small molecule-induced phospholipidosis in cells

This guideline describes the protocol of using high-content imaging to evaluate small molecule-induced phospholipidosis in cells, which might be an undesirable mechanism of action (MOA) of nuisance compounds in cell-based assays. The protocol has been applied for U2OS and A549 cells but can be adapted to other cell lines.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2025-02-14
DOI
https://doi.org/10.5281/zenodo.14872165
Primary Topic
Lysosomal Storage Disorders Research
Type
article
Field-Weighted Citation Impact
0.00
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article

REMEDi4ALL - Standard Operating Procedure - A high-content screening assay to evaluate small molecule-induced phospholipidosis in cells

Zenodo (CERN European Organization for Nuclear Research)
Lysosomal Storage Disorders Research
article

REMEDi4ALL - Standard Operating Procedure - A high-content screening assay to evaluate small molecule-induced phospholipidosis in cells

article en

Abstract

This guideline describes the protocol of using high-content imaging to evaluate small molecule-induced phospholipidosis in cells, which might be an undesirable mechanism of action (MOA) of nuisance compounds in cell-based assays. The protocol has been applied for U2OS and A549 cells but can be adapted to other cell lines.

Zenodo (CERN European Organization for Nuclear Research)
Karolinska Institutet (SE)
Industry, innovation and infrastructure
Openalex Percentile: Top 43%
Lysosomal Storage Disorders Research
DOIOpen Access PDFOpenAlex
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REMEDi4ALL - Standard Operating Procedure - A high-content screening assay to evaluate small molecule-induced phospholipidosis in cells · Zenodo (CERN European Organization for Nuclear Research) (2025) | TGRS Research Map | TGRS