Характеристика синтезу білка при експериментальному аутоімунному гепатиті на тлі введення кондиціонованого середовища мезехімальних стовбурових клітин

Characteristics of Protein Synthesis in Experimental Autoimmune Hepatitis Following Administration of Conditioned Medium from Mesenchymal Stem Cells Fedir Hladkykh ABSTRACT. The liver performs key functions in the body, including the synthesis of plasma proteins such as albumins and globulins, which are important for metabolic processes and drug binding. Protein synthesis disorders can be caused by liver diseases, particularly autoimmune hepatitis (AIH). Modern biotechnological approaches, including the use of conditioned medium from mesenchymal stem cells (MSC-CM), offer new prospects for treating such diseases. The efficacy of MSC-CM in AIH was studied in 42 male rats weighing 200–220 g. AIH in rats was induced by administering a hepatotropic antigenic mixture composed of Freund's complete adjuvant and an antigen solution obtained from an allogeneic liver homogenate. Treatment with MSC-CM resulted in a statistically significant (p<0.001) increase in the total protein level by 20.2% compared to the control group, reaching 72.1±1.6 g/L, which was 14.3% statistically significantly higher (p=0.01) than the value in rats treated with the reference drug, Silibor. Evaluation of the change in total protein levels by fractions showed that, following MSC-CM administration, the albumin fraction increased by 48.9% (p<0.001), while the globulin level increased by only 1.3% (p=0.9). KEYWORDS: autoimmune hepatitis, total protein, albumins, globulins, silibor. Гладких ФВ. Характеристика синтезу білка при експериментальному аутоімунному гепатиті на тлі введення кондиціонованого середовища мезехімальних стовбурових клітин. Матеріали ІІІ Міжнародної науково-практичної конференції «Молодіжна наука заради миру та розвитку»; 12–14 грудня 2024 р.; Чернівці: Чернівецький національний університет імені Юрія Федьковича МОН України; 2024, с. 47–50. DOI: https://doi.org/10.5281/zenodo.14941003