TANNIC ACID EXHIBITS NEUROPROTECTIVE ABILITIES IN THE MICROSTRUCTURES OF THE PREFRONTAL CORTEX OF ADULT WISTAR RATS FOLLOWING ETHANOL-INDUCED TOXICITY

Abstract: Background: High alcohol intake is a known cause of brain damage. Tannic acid (TA) poses as a potential treatment measure. Aim: To investigate the histomorphological changes in the microstructures of the prefrontal cortex of adult Wistar rats during treatment with tannic acid following ethanol-induced neurotoxicity. Methodology: Thirty six (36) adult male Wistar rats (160g-240g) were assigned into six (6) groups (A to F) of 6 rats each. Group “A” (untreated negative control) received daily doses of distilled water at 6ml/kg/bwt while Group “B” (alcohol control group) received daily doses of 6g/kg/bwt of 40% ethanol only. Group “C, D and E” received daily doses of 6g/kg/bwt of 40% ethanol co-administrated with 200mg/kg/bwt, 100mg/kg/bwt and 50mg/kg/bwt of TA respectively. Group “F” (positive control group) received daily doses of 6g/kg/bwt of 40% ethanol co-administrated with 335mg/kg/bwt of Vitamin E. All treatments were oral and lasted 14 days. The animals were sacrificed under intraperitoneal ketamin/thiopental sodium as anaesthesia after 24 hours of fasting. Brain tissues were quickly but carefully removed and fixed in 10% formal saline. The prefrontal cortex was grossed and processed according to standard protocols, sectioned at 5μm thick for standard histological studies. Result: Histomorphological analysis demonstrates that high and medium doses of TA preserve neuronal integrity and mitigate the damaging effects of ethanol exposure. Treatment with vitamin E suggests that while antioxidant properties may aid in neuronal preservation, they do not fully address the inflammatory responses triggered by ethanol. Conclusion: Tannic acid exhibits comparatively better neuroprotective effects against ethanol-induced prefrontal cortex toxicity. Keywords: Tannic acid, Ethanol toxicity, Prefrontal Cortex, Histomorphology, Wistar rats. Title: TANNIC ACID EXHIBITS NEUROPROTECTIVE ABILITIES IN THE MICROSTRUCTURES OF THE PREFRONTAL CORTEX OF ADULT WISTAR RATS FOLLOWING ETHANOL-INDUCED TOXICITY Author: Christian Chiemeka Ozor, Chike Anibeze, Elizabeth Finbarrs-Bello International Journal of Healthcare Sciences ISSN 2348-5728 (Online) Vol. 12, Issue 2, October 2024 - March 2025 Page No: 57-64 Research Publish Journals Website: www.researchpublish.com Published Date: 17-December-2024 DOI: https://doi.org/10.5281/zenodo.14509552 Paper Download Link (Source) https://www.researchpublish.com/papers/tannic-acid-exhibits-neuroprotective-abilities-in-the-microstructures-of-the-prefrontal-cortex-of-adult-wistar-rats-following-ethanol-induced-toxicity