ANALYSIS OF THE MICROBIOTA OF THE ORAL MUCOSA IN CHILDREN AND ADOLESCENTS WITH CHRONIC FORMS OF GINGIVITIS

Relevance. The pathogenic potential of some representatives of the oral microbiota is quite pronounced, which can be realized under various conditions both from the transient flora and from the resident flora. The researchers believe that the identified qualitative and quantitative shifts in the automicroflora of the oral cavity in children with CCG can lead to a long course of the disease, frequent relapses and chronicity of the process. Material and methods. A total of 425 children and adolescents were selected; including 195 (45.9%) with CHG (1 group) and 165 (38.9%) with CHG (2 groups) and 65 (15.3%) practically healthy (control group (CG) on the part of the TP, they were also divided into age groups and by gender. A survey, an objective examination of the PR, generally accepted clinical laboratory and dental research methods were conducted. Microbiological examination of biological samples, in particular, primary cultures for aerobic and facultative anaerobic microorganisms were carried out using selective and enriched nutrient media. The study of biological (biochemical) characteristics and pathogenicity factors of the isolated isolates was carried out by both classical and modern methods (bacteriological analyzer-Vitek 2 Compact; mass spectrometry -MALDI-TOF MS). Results. It was established that, according to the complaints of patients, 1-group. There was bleeding gums in 63.6%, while bleeding gums manifested in 6.1% of children when brushing their teeth, the presence of tartar (TC) was found in 23.03% of patients. In children and adolescents of the 2-group, inflammatory processes in the gums were noted in 67.9%, 80.1% of patients complained of pain and bleeding gums when brushing their teeth; the presence of tartar was found in 35.4% of patients. As studies have shown, the main microorganisms of the gingival sulcus and oral fluid were β-hemolytic streptococci of group A. At the same time, there was a significant increase in the number of representatives of the genus Neisseria spp., such as Neisseria mucosa, Neisseria sicca, Neisseria flavescens from 105-6 to 1012-13 CFU / ml, which indicates their leading place. With CCG and CHG, there was a significant increase in the proportion of representatives of the genus Candida spp., among which Candida albicans were detected reliably more often, by 1.8 and 1.4 times, respectively. Conclusion. The prevalence of CCG and CHG in children and adolescents aged 6 to 18 years is characterized by high incidence, while CCG and CHG make up the main proportion of CHG. Also, the dependence of the occurrence of these pathologies on the age and hygienic state of the PR, as well as the role of dental plaque as an etiological factor of the disease under study, is confirmed.