Remineralization Potential of Self-Assembling Peptide P11-4, 2% Arginine Enriched Sodium Fluoride and Functionalized Tri Calcium Phosphate Fluoride Varnishes in Depth of Artificial Carious Lesion: Polarized Light Microscopic Evaluation

Background: White spot lesions (WSLs) known as hidden caries. Aim: toevaluate the remineralizing potential of Self-assembling peptide P11-4, 2%Arginine Enriched Sodium Fluoride varnish and Functionalized Tri CalciumPhosphate Fluoride varnish regarding artificially produced carious lesionsutilizing polarized light microscope (PLM). Methods: Thirty-two bovineenamel samples were prepared and artificially demineralized by submersionin an acidic solution (pH 4.4) for four days until a homogenous white spotlesion was formed and observed when the specimen dried. They wererandomly located into four groups: (A): Self-assembling peptide P11-4varnish; (B): 2% Arginine Enriched sodium fluoride varnish; (C): Tri-CalciumPhosphate Fluoride Varnish; and group (D): Untreated negative control. Allsamples underwent a standardized protocol of regulated pH cycling for 12days. The extent of remineralization for each drug was evaluated using PLM.Results: All three examined materials demonstrated enhancement in the depthof artificial carious lesions. In comparison to the negative control group D(20.69±1.98), both groups B (80.22±9.18) and C (74.93±14.43) exhibited astatistically significant decrease in lesion depth relative to group A(34.45±12.73). Conclusions: All tested materials showed improvement in thelesion depth. Arginine containing fluoride and Tri Calcium Phosphate fluoridevarnish showed better results than self-assembling peptide.