A guide to selecting high-performing antibodies for GPNMB (UniProt ID: Q14956) for use in western blot, immunoprecipitation, and immunofluorescence

This report provides a guide for selecting high-quality commercial antibodies against the transmembrane glycoprotein NMB (GPNMB) for applications in western blot, immunoprecipitation, and immunofluorescence, using a knockdown cell line as an isogenic control. This research was partially supported by the Emory-Sage-SGC TREAT-AD center, established by the National Institutes of Aging under award number U54AG065187.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2024-12-11
DOI
https://doi.org/10.5281/zenodo.14393182
Primary Topic
Monoclonal and Polyclonal Antibodies Research
Type
article
Field-Weighted Citation Impact
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article

A guide to selecting high-performing antibodies for GPNMB (UniProt ID: Q14956) for use in western blot, immunoprecipitation, and immunofluorescence

Zenodo (CERN European Organization for Nuclear Research)
Monoclonal and Polyclonal Antibodies Research
article

A guide to selecting high-performing antibodies for GPNMB (UniProt ID: Q14956) for use in western blot, immunoprecipitation, and immunofluorescence

article en

Abstract

This report provides a guide for selecting high-quality commercial antibodies against the transmembrane glycoprotein NMB (GPNMB) for applications in western blot, immunoprecipitation, and immunofluorescence, using a knockdown cell line as an isogenic control. This research was partially supported by the Emory-Sage-SGC TREAT-AD center, established by the National Institutes of Aging under award number U54AG065187.

Zenodo (CERN European Organization for Nuclear Research)
Montreal Neurological Institute and Hospital (CA)
Industry, innovation and infrastructure
Openalex Percentile: Top 46%
Monoclonal and Polyclonal Antibodies Research
DOIOpen Access PDFOpenAlex
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A guide to selecting high-performing antibodies for GPNMB (UniProt ID: Q14956) for use in western blot, immunoprecipitation, and immunofluorescence · Zenodo (CERN European Organization for Nuclear Research) (2024) | TGRS Research Map | TGRS