A guide to selecting high-performing antibodies for Rab7A (UniProt ID: P51149) for use in western blot, immunoprecipitation, and immunofluorescence

This report presents a guide to selecting high-quality commercial antibodies against GTPase Rab7A in western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic controls. This work was supported by the CQDM (by a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec) as well as the Government of Canada through Genome Canada, Genome Quebec, and Ontario Genomics (grant no. OGI-210).