CORRELATION OF RED BLOOD CELLS STORAGE LESION F2α-ISOPROSTANES LEVELS WITH ERYTHROCYTE-DERIVED MICROPARTICLES

Abstract Background: PRC stored blood products undergo changes in morphology, biochemistry, and metabolic called storage lesion red blood cells (erythrocytes storage lesion). Morphological changes of erythrocyte membrane phospholipids and damage due to oxidative damage and hemolysis allegedly produce microparticles known as erythrocyte-derived microparticles (EMP) and F2α-isoprostanes. F2α-isoprostanes are the indicators used to assess lipid peroxidation and cellular oxidative stress. Increased EMP-isoprostanes F2α in the PRC product allegedly one of the cause’s bad outcomes in transfusions. Aim: to know the correlation between red blood cells storage lesions (F2α-isoprostane) with erythrocyte-derived microparticles (CD235a) in the PRC product based storage time day 0, 7,14,21 and 28 at blood banks Hospital Dr. M. Djamil Padang. Methods: The cross-sectional study of 14 PRC units came from 14 heallthy donors at Blood Bank of Dr. M.Djamil Padang Hospital. The study lasted from the month of May 2016 to August 2017. The tests were conducted during 28 days of storage at 1-week intervals. Examination the level F2α-isoprostane with methods Enzyme-linked immunosorbant assay (ELISA) and examination of EMP levels (CD235a) with flowcytometry method. Bivariate statistical analysis of the correlation parameter storage lesion red blood cells (F2α-isoprostanes) with EMP (CD235a) using Pearson correlation test with a P value <0.05 indicates exhibited significantly. Results: Fourteen units of PRC obtained the largest donors were male (85.7%) with the highest blood type is O (42.9%). During storage there is an increased the amount of F2α-isoprostane and erythrocyte-derived microparticles (EMP) in the PRC product. There was no statistically significant correlation between the level F2α-isoprostane F2α and the amount of EMP in the during storage up to 28 days. Conclusion: There was no statistically significant correlation between the level-isoprostane F2α and the amount of EMP in the storage PRC.