Research on Synergestic Anthelmintic Activity of Methanolic Extract of the Leaves of Cyclea Peltata Lam and Roots of Cocos Nucifera Linn Against Adult Pheretima Posthuma Worms and it's Statistical Analysis

ABSTRACT : The aim of the research is to develop a safe and effective anthelmintic from plants, due to the increasing toxic effect of synthetic drugs. The reason behind this research is the undesirable side effects of synthetic drugs available in the market like drug resistance. So there is a need for the exploration of more medicinal plants for the treatment of various worm infections. Here the anthelmintic activity of combined methanolic extract of leaves of Cyclea peltata and roots of Cocos nucifera were studied. The extract were prepared by maceration technique and evaluated the anthelmintic activity against adult Pheretima Posthuma worms via anthelmintic assay. The reason for selecting these two plants for monitoring the anthelmintic activity because of the presence of tannins, which have been proved by previous phytochemical studies. Tannins have the capability to bind free proteins present in the cuticle, oral cavity etc, thus causing starvation of the larvae and thus possibly resulting in their death. Combined Anthelmintic activity of the methanolic extract of the leaves of Cyclea peltata and roots of Cocos nucifera were carried out by one way ANOVA followed by Tukey’s multiple comparison test at 95% confidence interval using graph pad prism software version 9.0.0 Statistical analysis also shows that the combined extracts increase the anthelmintic activity by each other, which can be monitored by analyzing the statistical significance and statistical non-significance, thus it proved to be synergistic. About three formulations were prepared from combined plant extract (25,50 and 100mg/5ml) and the time of paralysis and death were calculated, which establish significant synergistic anthelmintic activity against adult Pheretima Posthuma worms. Albendazole 25mg/5ml were used as a standard drug. Thus the plants can be used for the safe and effective management of helminthiasis.