Investigation of potential antioxidant and anti-urolithiatic activity from methanolic extract of Atriplex halimus

The present work has for objective to evaluate the antioxidant and antiurolithiasic and activities of the methanolic extract AHMeOH leaves of the plant Atriplex halimus. In vitro evaluation of the antioxidant activity of the extract was carried out by tthe method the reducing power assay. The method of reducing power assay shows an increase in absorbance with different concentrations of the extract compared to the negative control with a significant difference (P≤0.002). The concentration (2mg/ml) gives the antioxidant best activity compared to other concentrations with a highly significant difference (P≤0.05) . In antiurolithiasise activity, six groups of Wistar rats were treated respectively with distilled water, 1% ethylene glycol in distilled water, AHMeOH (100 mg/Kg bw), AHMeOH (200 mg/kg bw) beginner the first day, AHMeOH (100 mg/Kg),AHMeOH (200 mg/kg bw) after 15 days of ethylene glycol adminstration. Just after day 28 of experiment, urine samples were collected and the volume, the pH was measured. After, were analyzed for the following dosages: urinary oxalate as well as blood samples are taken at the ocular sinus after anesthesia for the determination of: urea, creatinine and uric acid. The results show that the control group has severe damage while the groups treated with AHMeOH reduced significantly (P≤0.05) the formation of stones in the kidneys.