Constant enzymatic in situ production of H₂O₂ for an Unspecific Peroxygenase by an L-amino acid oxidase

Unspecific Peroxygenases have been the subject of a lot of research in the recent past as they offer opportunities for green hydroxylation reactions using hydrogen peroxide (H2O2) as donor. A striking problem of UPOs is their low stability against their own co-substrate H2O2. In order to prevent the deactivation, we developed an enzyme cascade with an l-amino acid oxidase (LAAO) to produce hydrogen peroxide in situ at a constant low concentration, aiming for much higher lifespan and therefore longer working time of the enzyme. This way, we are able to convert a variety of substrates with conversion rates up to 95%.