Arabidopsis-FCN

Functional carbon-based nanomaterials (FCNs) are novel carbon-based nanomaterials with functional groups attached to the sp2 hybridized carbon backbone and carbon-based small-molecule compounds. Addition of FCNs to growth medium has demonstrated growth promoting effects on the model plant species Arabidopsis thaliana including accelerated vegetative growth, increased leaf chlorophyll contents, and enhanced root activity. The time-course RNA-sequencing (RNA-seq) data hereby deposited at CNGB provide snap shots of the transcriptomic dynamics in A. thaliana leaf tissues in response to FCN treatment, representing the first step towards dissecting the physiological mechanism underlying the FCN-induced growth promotion effect on Arabidopsis and other plant species. Specifically, the compressed fastq files contain raw pair-end RNA-seq reads generated on the Illumina NovaSeq platform from Arabidopsis leaf tissue cultivated on Murashige-Skoog media with or without FCN addition for 2 or 6 hours. Untargeted analyses of differentially expressed genes (DEGs) reveal that FCN induces widespread changes in the transcriptomic landscape, with disproportional promoting effects on photosynthesis- and cellular transport-related genes while suppressing genes involved in DNA repair and translation process. Targeted analyses of genes involved in specific phytohormonal signaling pathways suggest that FCN may accelerate vegetative growth by promote cell expansion regulated by the auxin/gibberellic acid/brassinosteroid transcriptional regulatory module.