Rutin Effects on Human Senescent Stromal Cells Function: a Network Analysis Study

Background and objectives: Rutin is a lipophilic natural flavonoid. It is found in vegetables, citrus fruits, and beverages. This study aims to evaluate rutin metabolic pathways in human senescent stromal cells. Methods: Data are extracted from the Gene Expression Omnibus (GEO) database and pre-evaluated via GEO2R to find the significant differentially expressed genes (DEGs). The significant DEGs were assessed via protein-protein interaction PPI network analysis to explore the hub genes. The hubs were screened via directed PPI to find the critical DEGs. Results: Data were assessed via volcano plot, Uniform Manifold Approximation and Projection (UMAP) plot, and Venn diagram. A total number of 9124 significant DEGs were analyzed to determine 33 upregulated and 61 downregulated hubs. The identified hubs were investigated via directed PPI and Il1B. ICAM1, CCL2, EGF, CXCL8, PTGS2, CAMK2B, CCN2, VCAM1, ELN, CXCL12, BGN, and TLR4 were pointed out as critical hub genes. Conclusion: Il1B, CCL2, GNAO1, ICAM1, EGF, and CXCL8 appeared as controller genes affected by rutin while PTGS2 and CAMK2B were the most controlled individuals. The finding refers to the significant advantages of the rutin effect on the function of treated cells. These advantages are corresponded to the usefulness of rutin as an herbal drug candidate. However, more investigations are required to decrease its side effects.