Nanotechnology - Enhanced CRISPR-Cas9 Epigenome Editing for Cancer Therapy: Innovations in Delivery, Targeting and Safety

Study Overview: This systematic review examines the integration of nanotechnology with CRISPR-Cas9 systems for targeted epigenome editing in cancer therapy. The study addresses critical gaps in current research by evaluating how various nanoparticle-based delivery systems enhance the precision, efficacy, and safety of CRISPR-Cas9 applications for cancer treatment. Research Objectives: Examine the role of nanotechnology in enhancing CRISPR-Cas9 delivery and specificity for epigenome editing in cancer therapy Evaluate recent nanotechnology-based innovations aimed at reducing systemic toxicity and improving safety profiles Assess targeting strategies and therapeutic outcomes across different cancer models Methodology: Systematic literature search across PubMed, Scopus, and Nature databases (2014-2024) Keywords: "CRISPR-Cas9 epigenome editing", "nanotechnology-enhanced CRISPR delivery", "cancer-targeted epigenome editing", "CRISPR-Cas9 safety in cancer therapy" Focus on peer-reviewed articles with empirical data on safety, efficacy, and delivery mechanisms Key Findings: The review identifies five major nanoparticle platforms: Lipid Nanoparticles (LNPs) with enhanced endosomal escape capabilities Polymeric Nanoparticles (PLGA) with tumor-targeting ligands Gold Nanoparticles (AuNPs) combining photothermal therapy with gene editing Metal-Organic Frameworks (MOFs) enabling high-capacity intracellular delivery Magnetic Nanoparticles (MNPs) providing externally controlled targeting Clinical Relevance: This research contributes to developing safer, more precise cancer therapies by addressing delivery challenges and off-target effects that limit clinical translation of CRISPR-Cas9 technology. Publication Status: Published in Proceedings of the 15th International Conference on Sustainable Built Environment (ICSBE) 2024 Data Sharing: This project contains the complete methodology, data extraction protocols, analysis frameworks, and supplementary materials used in the systematic review to ensure reproducibility and transparency in research.