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<p><b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0311669#pone.0311669.g002" target="_blank">Fig 2A</a>.</b> Digital scan of autoradiogram of TLC plate. <sup>14</sup>C-Palmitate incorporation into lipids. Cell surface and cellular lipids from log-phase and 3-day biofilm cells of <i>M</i>. <i>abscessus</i> metabolically radiolabeled with <sup>14</sup>C-palmitate for 6 h in the presence or absence of NMP were analyzed by silica-TLC using chloroform:methanol:water (65:25:4, v/v/v) as solvent system. Autoradiogram of TLC plate from a representative experiment shown. Equal amounts of total lipid radioactivity loaded in each lane. Three independent experiments performed. Red boxes indicate lipids that showed significant changes upon NMP treatment across experiments. SF, solvent front; GPL, glycopeptidolipids (R<sub>f</sub> ~0.84); PE, phosphatidylethanolamine (R<sub>f</sub> ~0.48); CL, cardiolipin (R<sub>f</sub> ~0.36); PI, phosphatidylinositol; PIMs, phosphatidylinositol mannosides (R<sub>f</sub> ~0.26–0.1); Ori, origin/ sample loading zone. <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0311669#pone.0311669.g003" target="_blank">Fig 3A</a>.</b> Digital scans of autoradiograms of TLC plates. <sup>14</sup>C-Acetate incorporation into surface and cellular lipids from log-phase and 3-day biofilm cells of <i>M</i>. <i>abscessus</i> metabolically radiolabeled with <sup>14</sup>C-acetate in the presence or absence of NMP. Autoradiogram of TLC plate from a representative experiment shown. Equal amounts of total lipid radioactivity loaded in each lane. Three independent experiments performed. Cell surface and cellular lipids of log-phase and biofilm cells of <i>M</i>. <i>abscessus</i> were extracted and analyzed by silica-TLC using chloroform:methanol:water (65:25:4, v/v/v) as solvent system. SF, solvent front; GPL, glycopeptidolipids; PE, phosphatidylethanolamine; CL, cardiolipin; PI, phosphatidylinositol; PIMs, phosphatidylinositol mannosides; Ori, origin/ sample loading zone. <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0311669#pone.0311669.g004" target="_blank">Fig 4A</a>.</b> Digital scans of autoradiograms of TLC plates. Metabolic radiolabeling of the neutral storage lipid triacylglycerol (TAG) in the cellular lipids of log-phase and 3-day biofilm cells of <i>M</i>. <i>abscessus</i> with <sup>14</sup>C-palmitate for 6 h or <sup>14</sup>C-acetate for 24 h in the presence or absence of NMP was analyzed by silica-TLC using hexane:diethylether:acetic acid (80:20:2, v/v/v). Autoradiogram of TLC plate from a representative experiment shown. Equal amounts of total lipid radioactivity loaded in each lane. SF, solvent front; TAG, triacylglycerol (R<sub>f</sub> ~0.36); FA, fatty acid (R<sub>f</sub> ~0.13); Ori, origin/ sample loading zone.</p> <p>(PDF)</p>