Effect of using Pseudomonas fluorescens bacteria, Glomus mosseae fungus and liquid organic fertilizer on soil available nitrogen and phosphorus and some characteristics of fenugreek (Trigonella foenum graecum L.) and choline seed content

A factorial pot experiment was conducted during the fall season of 2023–2024 in the fields of the College of Agriculture/University of Wasit, according to a randomized complete block design (RCBD) with three factors and three replicates. The first factor is the bacterial inoculum Pseudomonas fluorescens at two levels (addition B1 and without addition B0), the second factor is the fungal fertilizer Glomus mosseae at two levels (addition M1 and without addition M0), and the third factor is the liquid organic fertilizer at two levels as well (addition O1 and without addition O0) added in two batches, thus we have eight treatments with three replicates so that the number of experimental units is 24 experimental units (pots). Available nitrogen and phosphorus in the soil, plant height, number of branches, number of pods, number of seeds per pod, seed content of active substance choline, and biological yield were measured. The triple interaction treatment of the three factors (B1M1O1) gave the best results for the mentioned traits, followed by the dual interaction treatment between bacteria and fungi (B1M1), then the dual interaction treatments between bacteria and liquid organic fertilizer B1O1 and between fungi and liquid organic fertilizer M1O1, then the single treatments B1, M1, and O1, and finally the control treatment without additives (B0M0O0), which gave the lowest results. The mycorrhizal reliability was calculated for each of the plant heights, number of pods, and biological yield and was 22.2, 68.25, and 33.00%, respectively.