Densities of <i>Wolbachia w</i>AlbA and <i>w</i>AlbB strains in the bodies of <i>Aedes albopictus</i> mosquitoes according to vector competence parameters represented by IDT scores.

<p>These analyses were performed using individual mosquito of F<sub>0</sub> generation, belonging to the populations of Sainte-Marie (F0_SM), Saint-Gilles les Hauts (F0_SG), Saint-Philippe (F0_SPh), or Saint-André (F0_SA) and examined at 21 and 28 days after being exposed to infectious blood meals containing the DENV-1 strain. The densities of <i>Wolbachia w</i>AlbA (orange), or <i>w</i>AlbB (green), or <i>w</i>AlbTot (<i>w</i>AlbA + <i>w</i>AlbB) (violet) (violet) are given based on the ratio between the <i>Wolbachia</i> and RSP7 concentrations which provided the number of <i>Wolbachia</i> genomes relative to the <i>Ae</i>. <i>albopictus</i> genomes. We measured the density of <i>Wolbachia</i> in individuals (N = 75) classified according to their IDT scores (0, 1, 2 or 3) defined as follows: the IDT score 0 for mosquitoes with no infectious DENV-1 particles either in the body, head or saliva; the IDT score 1 for samples with only infected bodies; the IDT score 2 for mosquitoes with infectious particles in the bodies and the heads; and the IDT score 3 for mosquitoes with infectious DENV-1 particles in the bodies, heads and saliva. dpe = days post-exposure.</p> <p>(DOC)</p>

Publication Details

Journal
Figshare
Published
2024-09-19
DOI
https://doi.org/10.1371/journal.pone.0310635.s011
Primary Topic
Insect symbiosis and bacterial influences
Type
article
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article

Densities of Wolbachia wAlbA and wAlbB strains in the bodies of Aedes albopictus mosquitoes according to vector competence parameters represented by IDT scores.

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Insect symbiosis and bacterial influences
article

Densities of Wolbachia wAlbA and wAlbB strains in the bodies of Aedes albopictus mosquitoes according to vector competence parameters represented by IDT scores.

article en

Abstract

<p>These analyses were performed using individual mosquito of F<sub>0</sub> generation, belonging to the populations of Sainte-Marie (F0_SM), Saint-Gilles les Hauts (F0_SG), Saint-Philippe (F0_SPh), or Saint-André (F0_SA) and examined at 21 and 28 days after being exposed to infectious blood meals containing the DENV-1 strain. The densities of <i>Wolbachia w</i>AlbA (orange), or <i>w</i>AlbB (green), or <i>w</i>AlbTot (<i>w</i>AlbA + <i>w</i>AlbB) (violet) (violet) are given based on the ratio between the <i>Wolbachia</i> and RSP7 concentrations which provided the number of <i>Wolbachia</i> genomes relative to the <i>Ae</i>. <i>albopictus</i> genomes. We measured the density of <i>Wolbachia</i> in individuals (N = 75) classified according to their IDT scores (0, 1, 2 or 3) defined as follows: the IDT score 0 for mosquitoes with no infectious DENV-1 particles either in the body, head or saliva; the IDT score 1 for samples with only infected bodies; the IDT score 2 for mosquitoes with infectious particles in the bodies and the heads; and the IDT score 3 for mosquitoes with infectious DENV-1 particles in the bodies, heads and saliva. dpe = days post-exposure.</p> <p>(DOC)</p>

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Insect symbiosis and bacterial influences
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