Gating strategy for sorting of TBKBP1-overexpressing CD8<sup>+</sup> T cells and EV-transduced controls.

<p>PBMCs isolated from healthy CMV-seronegative donors were stimulated with plate-bound anti-human CD3 and anti-human CD28 antibodies and subsequently co-transduced with mTCR and TBKBP1- or EV-mCherry plasmids. Successfully transduced CD8<sup>+</sup>mTCR<sup>+</sup>mCherry<sup>+</sup> T cells were sorted using flow cytometry. Representative flow cytometry plots from 3 independent donors show the gating strategy for sorting of CD8<sup>+</sup>mTCR<sup>+</sup>mCherry<sup>+</sup> T cells and post-sort purity.</p> <p>(PDF)</p>