Primer site binding mismatches for species-specific C. tumulosa primers.

Species-specific primer binding site mismatches for the chloroplast ribulose bisphosphate carboxylase large chain (rbcL) gene designed to exclusively amplify DNA from <i>Chondria tumulosa</i> (GenBank ID: MT039604). Target templates were matched <i>in silico</i> using the National Center for Biotechnology Information (NCBI) nucleotide collection (nt) Primer-BLAST tool (<a href="https://www.ncbi.nlm.nih.gov/tools/primer-blast/" target="_blank">https://www.ncbi.nlm.nih.gov/tools/primer-blast/</a>) or <i>in situ</i> using high-throughput sequencing of environmental DNA from water samples. Primer binding site mismatches are highlighted. (DOCX)