Variability of outcomes and noise in CSF ED assays.

<p>A(i)-C(i) Outcomes from three independent 2F4R ED assays performed separately for CSF dilutions (PD6) are shown. Black arrows show ThT outcome at two different dilutions (broken red circles, 1/2<sup>2</sup> and1/2<sup>5</sup>) tested in 3 independent ED assays. A(ii)-(iii), B(ii)-(iii), and C(ii)-(iii) show ThT outcomes (colored traces) for individual quadruplet wells for the tested CSF dilution (ii, 1/2<sup>2</sup>; iii, 1/2<sup>5</sup> in each case). Healthy CSFs tested in quadruplicate wells were used as controls. A(iv)-(v), B(iv)-(v), and C(iv)-(v) show example ThT outcomes for quadruplet wells for tested healthy CSFs. In each case, ThT threshold (horizontal broken line) and time cut-off (vertical broken line) for deciding positive/negative wells are shown.</p> <p>(DOCX)</p>