Representative flow cytograms of CFSE-labeled <i>Naegleria fowleri</i> (green), HT1080 cells expressing tdTomato (red) and their co-culture after pre-incubation with different inhibitors (CK-666, wortmannin, piceatannol and PP2).

(A) Flow cytograms of CFSE-labeled <i>N. fowleri</i> incubated with selected concentrations of inhibitors for 3 hours, showing no changes in the gated amoeba population. (B) Flow cytograms of HT1080 tdTomato cells incubated with selected concentrations of inhibitors for 3 hours showing no changes in the gated mammalian cell population. (C) Flow cytograms of CFSE-labeled <i>N. fowleri</i> preincubated with inhibitors in co-culture with HT1080 tdTomato cells. Cytopathogenicity is indicated by the number of <i>Naegleria</i> with ingested cell parts, represented by the red fluorescence of the tdTomato (orange) after 3 hours of co-culture. Preincubation with DMSO was used as a control. (PDF)