Combined effect of <i>NCP1</i> overexpression and <i>KSR1</i> LOH1.

<p>(A) OD600 values for liquid culture growth assays are plotted over time for the wild type progenitor (black), a strain engineered to contain a tet-off-NCP1 allele (light green) and a strain engineered to contain both the tet-off-NCP1 allele and the <i>KSR1</i> LOH1 (dark green). Growth in rich media without drug, 1 μg/mL FLC, or 256 μg/mL FLC are shown. Error bars are standard error for three replicates. MIC50 and SMG values calculated at 24 and 48 hours are shown to the left (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012497#sec011" target="_blank">Methods</a>). (B) As in (A), OD values for liquid culture growth assays are plotted over time for the wild type progenitor (black), a strain engineered to contain a tet-off-<i>NCP1</i> allele (light green), and the same strain grown in the presence of doxycycline (orange). Growth in rich media, 1 μg/mL FLC, and 256 μg/mL FLC are shown.</p> <p>(PDF)</p>