Long-term co-culture of <i>Naegleria fowleri</i> with mammalian cells affects amoeba cytopathogenicity but has no effect on its proliferation rate and trogocytosis.

(A) Graph of doubling time of axenically cultured <i>N. fowleri</i> (axenic) and long-term co-cultured <i>N. fowleri</i> (co-cultured) in host cell-free medium. (B) Graphs showing changes in the percentage of CellTrace Far Red-labeled axenic or long-term co-cultured <i>N. fowleri</i> (Nf CelltraceFR) with ingested either cytosolic eGFP or membrane-targeted eGFP-CAAX from HT1080 cells after 3 hours of co-incubation as measured by flow cytometry. (** p-value<0.01) (C) Live imaging of CellTrace Far Red-labeled <i>N. fowleri</i> (magenta) and HT1080 fibrosarcoma cell with eGFP-CAAX (green) co-culture showing the ingestion of human cell plasma membrane by amoebae. Scale bar=10 µm. (PDF)