Parasite killing rate.

Rate of parasite killing for compounds <b>22</b>, <b>31</b> and <b>33</b> was examined by treating Dd2 parasites in unlabelled RBCs with 10×IC₅₀ for 24 and 48 h, followed by washout. Reinvasion of viable parasites into fresh RBCs pre-labelled with carboxylfluorescein diacetate succinimidyl ester (CFDA-SE) was measured after a further 48 h, and the percentage of remaining viable parasites compared with untreated controls. For comparison, standard antimalarials included fast-killing dihydroartemisinin, chloroquine and slow-killing atovaquone. The average (±SD) of three biological replicates (each with two technical replicates) is shown. (PDF)