The « arm » region from Nmd4 is sufficient for the interaction with Upf1-HD.

<p>(A) The Nmd4 « arm » domain is sufficient for the interaction with Upf1-HD in vitro. Pull-down experiments of Upf1-HD by different His-tagged Nmd4 domains. Input and elution (His) samples were analyzed on 12% SDS/PAGE and Coomassie blue staining. L: Molecular weight ladder. (B) The Nmd4 « arm » region is required for its interaction with Upf1 in vivo. Co-purification of Nmd4-FL-HA or Nmd4-PIN-HA with Upf1-FL-TAP was evaluated using an anti-HA immunoblot on total extracts and purified fractions. Strains expressing Nmd4-FL-HA and Nmd4-PIN-HA and native untagged Upf1 were used as negative controls.</p> <p>(PDF)</p>