Identification of auxin reporter genes for <i>Datisca glomerata</i>.

(A) A member of the Small <u>A</u>uxin-<u>U</u>p <u>R</u>NA family of <i>D</i>. <i>glomerata</i> (DgSAUR1) shows high sequence similarity with members of the orthologous group ENOG410J0BD along with proteins from <i>Parasponia andersonii</i> (PON39119.1) and <i>Medicago truncatula</i> (KEH36239.1). (B) Members of the <i>Gretchen Hagen 3</i> family are expressed in nodules of <i>D</i>. <i>glomerata</i>; DgGH3.1 and DgGH3.2 were compared by multiple sequence alignment with twelve sequences from the orthologous group ENOG410IHQ2 (B). To this set, previously characterized sequences from <i>Arabidopsis thaliana</i> were added: AtGH3.5/WES1 and AtGH3.1/JAR1. While AtGH3.5/WES1 has high affinity for both indole-3-acetic and salicylic acid (Westfall et al., 2016), AtGH3.11/JAR1 responds to jasmonic acid (Westfall et al., 2012). Illustrated blocks represent individual motifs to which a link of structural arrangement with function has been demonstrated (refer to Figures 2 and 3 of Westfall et al., 2016). Additional metrics like conservation, quality, consensus, and occupancy are given. (C) Genetic dependencies of these promoters to auxins on 54-day-old <i>D</i>. <i>glomerata</i> roots. <i>Y-axis</i> shows the mRNA quantity relative to that of <i>PUQ</i>, <i>EF1-α</i>, and <i>TIP41</i>. Phytohormones and molarities are given on the <i>X-axis</i>. Significant differences to the control are highlighted by Welch’s pairwise t-test with Holm’s correction at p<0.01 (**). Gene names are given. (PDF)