Bilateral crosses of <i>pph22</i>Δ, <i>far8</i>Δ, and <i>mob3</i>Δ mutants.

<p>Strains were grown in YPD overnight cultures before mixing cells in a 1:1 ratio for spotting onto MS medium. Each cross was spotted 10 times onto an MS plate, along with one H99α x KN99<b>a</b> wild-type control. Bilateral (mutant x mutant) crosses were incubated at room temperature (24°C) in the dark for 8 weeks. No signs of mating between <i>pph22</i>Δ, <i>far8</i>Δ, and <i>mob3</i>Δ strains, in both <b>a</b> and α mating types, were observed.</p> <p>(DOCX)</p>