Standard addition of synthetic 1-TbAd and 1-tuberculosinyladenine to measure their abundance in clinical isolates of Mtb.

<p>(A) The quantities of natural molecules were estimated by plotting of chromatogram area against the known concentrations of each synthetic compound to obtain the extrapolated number on the x-axis. The arrows pointed to the concentration of the natural molecules. (B) The amount of 1-TbAd and 1-tuberculosinyladenine measured in 8 independent clinical isolates relative to total cellular lipid measured on a balance. The measurements underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.s013" target="_blank">S1 Data</a>.</p> <p>(PDF)</p>