RT-qPCR for <i>CDR1</i> in <i>KSR1</i> LOH1 strain.

<p>(A) RT-qPCR data for one biological replicate in technical triplicate is shown for expression of the gene <i>CDR1</i> relative to the gene <i>ACT1</i> for the <i>KSR1</i> LOH1 engineered strain, the <i>KSR1</i> LOH1 engineered strain growth in 1 μg/mL FLC, and the progenitor strains grown in 1 μg/mL FLC, all relative to the WT (progenitor) strain grown in rich media. Error bars are standard deviations for technical triplicate with propagation of error during delta-delta CT calculations. (B) Data for the first biological replicate, calculated as in (A) but normalized to the gene <i>TEF1</i> rather than <i>ACT1</i>. (C) As in (A), RT-qPCR data for a second biological replicate in technical triplicate.</p> <p>(PDF)</p>