Ion chromatograms of Mtb H37Rv or engineered mutant strains with inset boxes showing select dithered peaks to distinguish the overlapping biological replicates.

<p>(A) 1- and <i>N</i><sup>6</sup>-TbAd (<i>m/z</i> 540.354) are shown in 3 experiments comparing Mtb H37Rv (black), Δ<i>Rv3378c</i> (red), and the complemented strain Δ<i>Rv3378c</i>::<i>Rv3378c</i> (blue) in a validation experiment (<i>n</i> = 4); or Mtb H37Rv, ΔRv3377c (orange; dashed), Δ<i>Rv3378c</i>, and ΔRv3377-8c (gold; dashed) two-gene deletion in an independent experiment (<i>n</i> = 4); or Mtb H37Rv and Δ<i>Rv3376</i> (green) in an analysis of <i>Rv3376</i> function (<i>n</i> = 3). (B) 1- and <i>N</i><sup>6</sup>-tuberculosinyladenine (<i>m/z</i> 408.312) measured in the Mtb H37Rv (black), Δ<i>Rv3378c</i> (red), and the complemented strain Δ <i>Rv3378c</i>::<i>Rv3378c</i> (blue). 1-TbAd (A) and 1-tuberculosinyladenine (B) area under the curve were used for statistical testing and to generate Figs <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.g001" target="_blank">1B</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.g003" target="_blank">3A</a>, respectively.</p> <p>(PDF)</p>