Influence of RNase III on cell viability and Yop protein secretion.

<p>(<b>A</b>) Viability of <i>Y</i>. <i>pseudotuberculosis</i> strains YPIII (wt), YP356 (Δ<i>rnc</i>), and YP356 (Δ<i>rnc</i>) pIVO20 (p<i>rnc</i><sup>+</sup>). Data represent the mean ± SD from four independent biological replicates (lower panel). No significant differences compared to the wildtype were determined using the Student’s t-test. (<b>B</b>) <i>Y</i>. <i>pseudotuberculosis</i> strains YPIII (wt), YP356 (Δ<i>rnc</i>), and YP356 (Δ<i>rnc</i>) pIVO20 (p<i>rnc</i><sup>+</sup>) were grown at 37°C for 4 h; the secreted proteins in the supernatant of the cultures were precipitated with TCA and separated on SDS gels. The Yop secretion-deficient mutant YP101 (Δ<i>yscS</i>) was used as a negative control.</p> <p>(PDF)</p>