Effect of Pectin Oligosaccharides from Hawthorn on the H2O2-induced Human Embryonic Lung Fibroblast Cells

In this study, the anti-aging effects and mechanism of action of pectin oligosaccharides from hawthorn (HPOS) on H2O2-induced human embryonic lung fibroblast cells were evaluated using the MRC-5 cell line. The effects on cell viability, survival rate, β-galactosidase activity, mitochondrial membrane potential, the content of reactive oxygen species (ROS), antioxidant capacity, and the expression levels of proteins associated with the p53/p21/p16 signaling pathway were evaluated. The findings illustrated that compared with the model group, under the intervention of HPOS at a concentration of 0.8 mg/mL, the cell viability, survival rate, and mitochondrial membrane potential of MRC-5 cells increased by 16.4%, 17.0%, and 284.3% (P<0.01) , respectively. Furthermore, the proportion of β-galactosidase-positive cells and fluorescence intensity of ROS significantly decreased by 66.9%，77.2% (P<0.01), respectively. The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) contents increased by 72.0% and 50.0%, respectively. Compared to that in the model group, the malondialdehyde (MDA) content decreased by 42.6% (P<0.01) significantly. HPOS at a concentration of 0.8 mg/mL significantly inhibited p53, p21, and p16 expression in the p53/p21/p16 pathway (P<0.01). Thus, HPOS ameliorates the H2O2-induced aging of MRC-5 cells by inhibiting apoptosis and oxidative stress damage along with regulating the p53/p21/p16 pathway, thereby providing a scientific basis for the development of HPOS as a functional food ingredient that acts against aging.