Examples of adapting and exiting cells.

<b>A)</b> Classification of cell behaviors based on Clb2 dynamics. From left to right, a cell defined as arrested, where Clb2 signal is never degraded; a cell partially degrading Clb2; and a cell fully degrading Clb2. Rightmost panel: barplots presenting the percentages of the different classes of cells in wild-type cells arrested in nocodazole indefinitely. <b>B)</b> Two examples of Mad2 Localization Index (black curve) and Clb2 mean signal (grey curve) over time. In the left panel, a cell defined as exiting: at the moment of Clb2 degradation (grey dot, dashed vertical line) Mad2 Localization Index lies below the threshold (dashed horizontal line). The threshold is defined based on the Localization Index value in G1. The green dot highlights that the value of Mad2 Localization Index at Clb2 degradation time is below the threshold. In the right panel, a cell defined as adapting. In this cell, Mad2 Localization value at the Clb2 degradation time is above the threshold. This is highlighted by the purple dot. These examples come from a washout experiment but the definition applies also to cells always kept in nocodazole. Details of the analysis are presented in the Image analysis section of the Materials and Methods. (PDF)