Successful gene disruption of <i>L</i>. <i>mexicana</i> CALP1 (LmxM.20.1180) with rRNA-P stable protocol.

<p>(A) CALP1 (LmxM.20.1180) gene is located in chromosome 20 and shares the conserved sequence with the downstream CALP2 (LmxM.20.11185) gene. (B) Strategy used in this study to disrupt CALP1 gene. On the left panel, a gRNA was designed to target the specific 5’ end coding sequence of CALP1 gene, which was then disrupted with the bleomycin resistance gene donor. On the right panel, PCR analysis shows both CALP1 gene alleles were successfully disrupted.</p> <p>(PDF)</p>

Publication Details

Journal
Figshare
Published
2024-12-30
DOI
https://doi.org/10.1371/journal.pone.0316331.s003
Primary Topic
Genetic Mapping and Diversity in Plants and Animals
Type
article
Field-Weighted Citation Impact
0.00
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article

Successful gene disruption of L. mexicana CALP1 (LmxM.20.1180) with rRNA-P stable protocol.

Figshare
Genetic Mapping and Diversity in Plants and Animals
article

Successful gene disruption of L. mexicana CALP1 (LmxM.20.1180) with rRNA-P stable protocol.

article en

Abstract

<p>(A) CALP1 (LmxM.20.1180) gene is located in chromosome 20 and shares the conserved sequence with the downstream CALP2 (LmxM.20.11185) gene. (B) Strategy used in this study to disrupt CALP1 gene. On the left panel, a gRNA was designed to target the specific 5’ end coding sequence of CALP1 gene, which was then disrupted with the bleomycin resistance gene donor. On the right panel, PCR analysis shows both CALP1 gene alleles were successfully disrupted.</p> <p>(PDF)</p>

Figshare
Openalex Percentile: Top 43%
Genetic Mapping and Diversity in Plants and Animals
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Successful gene disruption of <i>L</i>. <i>mexicana</i> CALP1 (LmxM.20.1180) with rRNA-P stable protocol. · Figshare (2024) | TGRS Research Map | TGRS