Methods used to increase <i>rad24</i>Δ and <i>mec1-mn</i> spore viability.

<p>All error bars are standard error of the mean. <b>A)</b> Rescue of <i>rad24</i>Δ spore viability by <i>sml1</i>Δ. The spore viability of both SK1 and SK1xS288c hybrid strains with a deletion of <i>RAD24</i> is improved by the additional deletion of <i>SML1</i>. <b>B)</b> The effect of increasing prophase length on the spore viability of hybrid <i>mec1-mn</i> and <i>rad24</i>Δ yeast. Hybrid SK1xS288c strains are arrested in prophase and released after 4–10 hours using an inducible <i>NDT80</i> system ‘<i>ndt80AR</i>’. Both Mec1 and Rad24 mutants display an improvement in spore viability when prophase is extended (compare with non-arrested viabilities shown in <b><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011485#pgen.1011485.g001" target="_blank">Fig 1C</a></b>), and the level of improvement correlates with the length of the arrest.</p> <p>(PDF)</p>