Nonaggressive, nonsocial triggers do not modulate subsequent interactions with females.

<p>(A) Schematic of nonaggressive, nonsocial sequences. Sequences consisted of a male walking in isolation followed by male–female social interactions. (B) The number of male–female interactions after walking or not walking. Lines connect co-recorded mice. Black lines and white boxes show the medians and interquartile ranges (25%–75%). Wilcoxon signed rank test, W = 47, <i>p</i> = 0.24. (C) The latency between walking or not walking behaviors and social interactions. Wilcoxon signed rank test, W = 21, <i>p</i> = 0.54. (D) The duration of social interactions following walking-triggered sequences. Wilcoxon signed rank test, W = 32, <i>p</i> = 0.68. (E) Performance of decoders when predicting the behavioral state of the male social partner in post-aggression social interactions. Black lines and white boxes show the means and standard deviations. The red line denotes chance levels. Each condition: 1-sided z-test, <i>n</i> = 1,000 iterations. Observed: z = 1.73, <i>p</i> = 0.04; size-matched: z = 1.11, <i>p</i> = 0.13; randomized: z = −0.31, <i>p</i> = 0.38. Numerical values for S4B–S4D Fig are available as an online supporting file (S1 Data). Source data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002496#pbio.3002496.s013" target="_blank">S1</a>–<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002496#pbio.3002496.s024" target="_blank">12</a> Datasets.</p> <p>(DOCX)</p>