Anatomopathological analysis of C57BL/6 mice inoculated intracerebrally with brain homogenate from a spontaneously sick TgMoL108I transgenic mouse.

Histopathological assessment of spongiform lesions and PrP^res deposits of C57BL/6 mice after serial inoculation (1^st and 2^nd passages) with brain homogenate from a terminal ill TgMoL108I mouse with spontaneous disease. Analysis revealed no spongiform changes upon hematoxylin and eosin staining (H&E), as shown in the thalamic region of representative animals. PrP^res deposits were undetectable, using 6C2 mAb (1:1,000), demonstrating that this spontaneously generated prion strain failed to transmit to wild type mice. H&E: Hematoxylin and eosin; IHC: Immunohistochemistry. (PDF)