S1 Fig -

<p>Qubit-based quantification of the DNA present after different steps (P1—P7) of the DNase treatment (A) and visualization of free DNA present after steps P1—P7 of the DNase treatment (B). The error bars indicate the standard deviation. Model 1 ANOVA: * = p < 0.05, ns = non-significant (p > 0.05). DNA gel electrophoresis on a 0.6% (w/v) agarose gel (120 V, 100 min). Samples were: P1 = untreated, P2 = incubation at 37°C, P3 = addition of reaction buffer and incubation at 37°C, P4 = incubation at 37°C and 65°C, P5 = addition of reaction buffer and incubation at 37°C and 65°C, P6 = addition of reaction buffer, incubation at 37°C, addition of stop buffer and incubation at 65°C, P7 = addition of 40 U DNase and reaction buffer, incubation at 37°C, addition of stop buffer and incubation at 65°C.</p> <p>(DOCX)</p>

Publication Details

Journal
Figshare
Published
2024-12-03
DOI
https://doi.org/10.1371/journal.pone.0313774.s001
Primary Topic
Genetic and Environmental Crop Studies
Type
article
Field-Weighted Citation Impact
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Abstract

<p>Qubit-based quantification of the DNA present after different steps (P1—P7) of the DNase treatment (A) and visualization of free DNA present after steps P1—P7 of the DNase treatment (B). The error bars indicate the standard deviation. Model 1 ANOVA: * = p < 0.05, ns = non-significant (p > 0.05). DNA gel electrophoresis on a 0.6% (w/v) agarose gel (120 V, 100 min). Samples were: P1 = untreated, P2 = incubation at 37°C, P3 = addition of reaction buffer and incubation at 37°C, P4 = incubation at 37°C and 65°C, P5 = addition of reaction buffer and incubation at 37°C and 65°C, P6 = addition of reaction buffer, incubation at 37°C, addition of stop buffer and incubation at 65°C, P7 = addition of 40 U DNase and reaction buffer, incubation at 37°C, addition of stop buffer and incubation at 65°C.</p> <p>(DOCX)</p>

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Genetic and Environmental Crop Studies
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S1 Fig - · Figshare (2024) | TGRS Research Map | TGRS