Quality control and protein expression of mass spectrometry.

(A-B) The figure shows the distribution of peptide length and the corresponding m/z (mass-to-charge ratio) for peptides with different charge states, (A) for protein, and (B) for phosphorylation. Each point represents a peptide, with the color indicating its charge state. The histograms above and to the right of the scatter plot represent the distributions of peptide length and m/z, respectively, for peptides with different charge states. (C-D) The principal components analysis (PCA) results of 4 Ctrl samples (blue) and 4 MAP4K4 KO samples (red) for protein (C) and phosphorylation (D) from mass spectrometry. (E) GO-BP analysis of differential phosphorylation level proteins between Ctrl and MAP4K4 KO samples. Selected GO terms with Benjamini-Hochberg-corrected p values < 0.05 (one-sided Fisher’s exact test) are shown. The dot size represented the number of genes. The color scale represented the adjusted p value. (F) Western blot (WB) for STAT5A and GAPDH in Ctrl and STAT5A overexpression cells (left), for STAT5B and GAPDH in Ctrl and STAT5B overexpression cells (right). (PDF)