S1 Fig -

<p>(A) Replicon GFP expression and trans-encapsidation efficiency in the presence of an RNA replication inhibitor. The ΔP1 and ΔLbdcap GFP replicons were transfected into cells with (green and orange lines) and without (purple and black lines) the presence of GuHCl, a replication inhibitor. The green MFI was read over time using the Incucyte S3 Live cell imager to measure GFP expression levels from both replicating RNA and from input-only translation. The data shown represent the mean from triplicate wells, and the error bars represent the SEM from 12 images. (B) Replicon GFP expression and trans-encapsidation efficiency in the presence of an RNA replication inhibitor. The readout of the second round of the trans-encapsidation assay, measured by counting the number of GFP foci, when the first round was carried out in the presence or absence of GuHCl. The data shown represent the mean from triplicate wells, and the error bars represent the SEM from 12 images. (C, D) Mean and Standard Error data for <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012283#ppat.1012283.s001" target="_blank">S1 Fig</a> A and B. (E) Representative images analysed using the Incucyte software to obtain the: MFI data at the point of harvest for: (i-vi) <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012283#ppat.1012283.s001" target="_blank">S1 Fig</a> A ΔP1 and ΔLbdcap GFP replicons, in the presence and absence of GuHCl, and the cell only and capsid-donor only controls; and (vii-xii) peak GFP object count data for <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012283#ppat.1012283.s001" target="_blank">S1 Fig</a> B ΔP1 and ΔLbdcap GFP replicons, following a transfection in the first round of the assay in the presence and absence of GuHCl, and the cell only and capsid-donor only controls.</p> <p>(PDF)</p>