Microscopy of fluorescently tagged cell division proteins.

3 representative images for each construct are shown with corresponding empty vector controls. (A) sfGFP::BlhA localizes at sites of division at the mid-cell at either existing or forming septa. (B) Fluorescently tagged SPOR-domain protein shows localization at the cell membrane and at septa for dividing cells. (C) Smc2::sfGFP expression leads to elongated cells, with fluorescently tagged proteins aggregating inconsistently within the cells. (D) Expression of Smc2 from a replicative plasmid produces elongated cells. All bars are 5 μm; GFP fluorescence/phase contrast channels merged in composite images for all except (D). (PDF)