Characteristic similarities of top genes.

(A) In silico knockout of ELANE and SYK in neutrophils and B cells, respectively. Bar plots display the affected neutrophil and B cell related terms ranked by significance. The dotted line represents the significance threshold. (B) The dot plot showed the NeuRGI score of the top 12 genes. Genes displayed in bold indicate those with limited reports on their involvement in neutrophil differentiation or function. (C) Expression of <i>MAP4K4</i>, FIG4, and <i>TIGAR</i> in different immune cells from ImmuNexUT. The number behind genes represents cell specificity Tau value. (D) Bar plot shows main gene pathways affected by OntoVAE <i>in silico</i> KO of <i>MAP4K4</i>, FIG4, and <i>TIGAR</i> in neutrophils. In the ‘positive regulation of myeloid leukocyte differentiation’ pathway, <i>MAP4K4</i> exhibits the lowest p value among the three genes. The dotted line represents the significance threshold. (E) Expression of <i>MAP4K4</i>, FIG4, and <i>TIGAR</i> in neutrophil differentiation. We set ‘time cut’ for cells at different differentiation stages, with HSC set as 1 and Neu as 5, and performed linear regression fitting for the expression of these 3 genes. R represents the Pearson correlation coefficient, and the p value was calculated by t-test. (PDF)