Rgf1 interacts with the cell end marker Tea4 and binds to phosphatidylinositol-4-phosphate through its PH domain.

<p>(A) Colocalization of Rgf1 and Tea4. Super-resolution radial fluctuations (SRRF) images of WT cells producing Tea4-GFP endogenously (green) and Rgf1-tdTomato from a plasmid under the control of its own promoter (red). (B) Coprecipitation of Rgf1 and Tea1. Cell extracts from cells producing Tea1-GFP, Rgf1-HA, and Tea1-GFP and Rgf1-HA were precipitated with GFP-trap beads and blotted with anti-HA or anti-GFP antibodies (co-immunoprecipitation and immunoprecipitation). Western blot was performed on total extracts to visualize total Tea1-GFP and Rgf1-HA levels (whole cell extracts). The data underlying the graphs shown in the figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002491#pbio.3002491.s014" target="_blank">S1 Data</a>.</p> <p>(PDF)</p>