Ambiguous blood matching caused by multiple blood transfusions resulting in anti-C, anti-JKb combined with Anti-Wra: a case report

Objective To study the reasons for ambiguous blood matching in a patient who received multiple blood transfusions, and explore how to reduce the risk of producing homologous antibodies in patients who received multiple blood transfusions. Methods The antibody specificity was detected by serum antibody screening and identification, polyethylene glycol(PEG) immunoglobulin assay and dimercaptoethanol method based on clinical data analysis. Results The unexpected antibodies in the patient's serum were anti-C and anti-Wra, and anti-Jkb was detected after using polyethylene glycol immunoglobulin enhancement experiments. The anti-Wra was detected as IgM by dimercaptoethanol method, with a titer of 16, anti-C of 8, and anti-Jkb<1. Conclusion One case of ambiguous blood matching caused by anti-C, anti-Jkb combined with anti-Wra was discovered. By strengthening the experiments, the detection efficiency of Kidd system antibodies was improved, enabling timely provision of matching blood to patients.