PIP2-conjugated agarose beads pull-down assays from nuclear lysates with added nuclear RNA extract upon different conditions.

<p>The PIP2 and empty control beads were incubated for 1 h at 4°C in nuclear lysates, washed, and subjected to WB detection of CAND1 protein. WB signals at each pull-down condition in every repetition were normalized to the signal at PIP2 pull-down upon RNA addition condition. Statistical analysis was performed using Student’s t-test (n = 3). Error bars correspond to SEM (NL–nuclear lysate, * P < 0.05, ** P < 0.001, *** P < 0.0005, **** P < 0.0001). The following treatments were used in the respective specimens as indicated in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011462#pgen.1011462.g004" target="_blank">Fig 4A and 4B</a>: the addition of 30 μg of nuclear RNA extract, 300 mM NaCl, 100 mM NH<sub>4</sub>OAc, 10% 1,6-hexanediol, and 10% dextran.</p> <p>(PDF)</p>