Macrophage contribution to AEV engulfment, depletion with clodronate liposomes and <i>irf8</i> morpholino.

<p>(A)The number of epithelial stem cells that undergo apoptosis up to 3 h post treatment. <i>n</i> = 5 larvae, DMSO. <i>n</i> = 6, MTZ. **** <0.0001 using a two-tailed <i>t</i> test. (B) The number of engulfment events of macrophages in the presence of apoptotic cells across an 8-h timespan. <i>n</i> = 8 larvae, DMSO. <i>n</i> = 6, MTZ. *** 0.0003 using a two-tailed <i>t</i> test. (C) Fixed quantifications of macrophage presence at 6 hpt with the treatment of dexamethasone. <i>n</i> = 19 for DMSO, <i>n</i> = 33 for MTZ, <i>n</i> = 33 for MTZ + 100 μm Dexamethasone. ** 0.0072, **** <0.0001. (D) Representative images of Tp63 positive basal epithelial stem cells after treatment with Dexamethasone. Scale bar = 50 μm. (E) Ablation of the macrophage lineage using clodronate liposomes (scale bar = 200 μm). (F) Depletion of the macrophage population using <i>irf8</i> morpholino (scale bar = 200 μm). (G) Quantifications of the number of macrophages at 4 dpf. <i>n</i> = 72 for Control MO/Mock injected and <i>n</i> = 53 for <i>irf8</i> morpholino. ****<0.0001 via an unpaired two-tailed test. (H) The number of proliferating cells in <i>irf8</i> morpholino-injected larvae after MTZ treatment to induce apoptosis. <i>n</i> = 27 for DMSO, <i>n</i> = 41 for Control MO/Mock injected + MTZ, and <i>n</i> = 45 for <i>irf8</i> morpholino + MTZ. **** <0.0001. A two-way ANOVA with a Tukey’s ad hoc test was performed to assess significance. Scale bar = 200 μm. The underlying data for the graphs in this figure can be found in S2 Data.</p> <p>(PDF)</p>